Journal: Open Life Sciences

Article Title: BRCA1 subcellular localization regulated by PI3K signaling pathway in triple-negative breast cancer MDA-MB-231 cells and hormone-sensitive T47D cells

doi: 10.1515/biol-2020-0054

Figure Lengend Snippet: The sequence of primers used in this study

Article Snippet: The rabbit anti-BRCA1 primary antibody (cat. #bs-0803R, Bioss Co., Beijing, China) was added and incubated for 20 min at room temperature.

Techniques: Sequencing

Journal: Open Life Sciences

Article Title: BRCA1 subcellular localization regulated by PI3K signaling pathway in triple-negative breast cancer MDA-MB-231 cells and hormone-sensitive T47D cells

doi: 10.1515/biol-2020-0054

Figure Lengend Snippet: The subcellular localization of BRCA1 was observed under a fluorescence microscope (400×). The Control group was untreated. The HRG group was treated with 30 ng/mL HRG for 24 h to activate the PI3K signaling pathway. The HRG + LY294002 group was treated with 30 ng/mL HRG for 24 h to activate the PI3K signaling pathway and then treated with 25 μM LY294002 for 48 h to inhibit the PI3K pathway. The HRG + ShRNA group was treated with 30 ng/mL HRG for 24 h to activate the PI3K signaling pathway and then transfected with shRNA to silence Akt. The red arrows indicate the localization of BRCA1 protein in cell nuclei, while the yellow arrows indicate that on the edge of nuclear membranes.

Article Snippet: The rabbit anti-BRCA1 primary antibody (cat. #bs-0803R, Bioss Co., Beijing, China) was added and incubated for 20 min at room temperature.

Techniques: Fluorescence, Microscopy, shRNA, Transfection

Journal: Open Life Sciences

Article Title: BRCA1 subcellular localization regulated by PI3K signaling pathway in triple-negative breast cancer MDA-MB-231 cells and hormone-sensitive T47D cells

doi: 10.1515/biol-2020-0054

Figure Lengend Snippet: The protein and mRNA expression of Akt and BRCA1 in MDA-MB-231 cells. Cell treatment and grouping were described above. The protein and mRNA expressions were detected with Western blot and RT-PCR, respectively. (a) The protein expressions of Akt and BRCA1 and their phosphorylated counterparts. The protein expressions of (b) Akt and (c) BRCA1 were quantified in relative to that of GAPDH. (d) The percentage of p-Akt in the total Akt protein. (e) The percentage of p-BRCA1 in the total BRCA1 protein. (f) The relative expression of Akt mRNA and (g) the relative expression of BRCA1 mRNA were also detected. All experiments were performed at least three times. * P < 0.05, compared with the control group; # P < 0.05, compared with the HRG group.

Article Snippet: The rabbit anti-BRCA1 primary antibody (cat. #bs-0803R, Bioss Co., Beijing, China) was added and incubated for 20 min at room temperature.

Techniques: Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction

Journal: Open Life Sciences

Article Title: BRCA1 subcellular localization regulated by PI3K signaling pathway in triple-negative breast cancer MDA-MB-231 cells and hormone-sensitive T47D cells

doi: 10.1515/biol-2020-0054

Figure Lengend Snippet: The protein and mRNA expression of Akt and BRCA1 in T47D cells. Cell treatment and grouping were described above. The protein and mRNA expressions were detected with Western blot and RT-PCR, respectively. (a) The protein expressions of Akt and BRCA1 and their phosphorylated counterparts. The protein expressions of (b) Akt and (c) BRCA1 were quantified in relative to that of GAPDH. (d) The percentage of p-Akt in the total Akt protein. (e) The percentage of p-BRCA1 in the total BRCA1 protein. (f) The relative expression of Akt mRNA and (g) the relative expression of BRCA1 mRNA were also detected. All experiments were performed at least three times. * P < 0.05, compared with the control group; # P < 0.05, compared with the HRG group.

Article Snippet: The rabbit anti-BRCA1 primary antibody (cat. #bs-0803R, Bioss Co., Beijing, China) was added and incubated for 20 min at room temperature.

Techniques: Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction